WebTranscription is a highly dynamic process that generates single-stranded DNA (ssDNA) in the genome as ‘transcription bubbles’. Here we describe a kethoxal-assisted single-stranded DNA sequencing (KAS-seq) approach, based on the fast and specific reaction between N 3-kethoxal and guanines in ssDNA.KAS-seq allows rapid (within 5 min), … Web10 jan. 2024 · KAS-seq is an N3-kethoxal–assisted single-stranded DNA sequencing approach that allows rapid, sensitive and genome-wide mapping of single-stranded DNA produced in situ using as few as 1,000 ...
N3-kethoxal and experimental evaluation of its selectivity, cell ...
Kethoxal (3-ethoxy-1,1-dihydroxy-2-butanone) is an organic compound that has antiviral and anaplasmosis properties. It also forms a stable covalent adduct with guanine, which makes it useful for nucleic acid structure determination. WebMethylene blue dot results are listed as a control. d, Dot blot of isolated total RNA from mES cells that were treated by N3-kethoxal with different periods, 1, 5, 10, 15 and 20 min. e, … haxby dental practice
APExBIO - N3-kethoxal
Webk (x_i,x_j) 通常叫做核函数,但在这里无妨称之为相似度函数。. 顺带再说一句,这个其实就是soft threshold,每个样本都用到了,但权重不一样,和Ridge的表现形式差不多。. 3. 线性回归. 这个可以好好解释下。. 设训练集为 (X,y) ,则回归参数是 \hat {\beta} = … Web5 feb. 2024 · N3-Kethoxal分子具有很好的细胞渗透性,加入细胞培养基后5分钟左右RNA的标记即可达到饱和(图1d),具有比其他分子更快的修饰效果【8】。 此外,该分子的 … Web23 mei 2024 · Libraries treated with N3-kethoxal, kethoxal-remove and no treat were prepared, and the RNAs are extracted and for sequencing. Contributor(s) Weng X, Gong J, Chen Y, Zhang QC, Zhou X, He C: Citation(s) 32015521: Submission date: Nov 02, 2024: Last update date: May 23, 2024: Contact name: Xiaocheng Weng: both team to score accumulator