Webgene, that encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements, and (II) a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an IFN-β minimal promoter fused to 5 copies of the NF-κB consensus WebMinimum of 1 year of Retail or Sales experience. Creative individual who demonstrates good judgement and is tuned-into the pulse of the business. Self-motivated, results oriented, …

THP1-Blue ISG Cells IFN Reporter THP1 monocytes InvivoGen

WebWe next conducted the above experiments in a THP-1 monocytic cell line that has a luciferase reporter gene under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated ... WebDec 15, 2024 · In this system, ISG54 minimal promoter in conjunction with five ISRE elicits transcription of secreted luciferase reporter gene upon stimulation of the type I IFN pathway, and IFN-related immune activation was measured based on luminescence. 24. As a result of the SAR study (Table 1. dennis mcshane architect

Interferon regulatory factor 3 plays a role in macrophage

WebTHP1 dual cells (Invivogen) featuring the secreted luciferase reporter gene, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements (ISREs, under zeocin antibiotic selection), and secreted embryonic alkaline phosphatase (SEAP) reporter gene, driven by an IFN-β minimal promoter fused to five ... WebThis composite promoter is comprised of five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter, which is unresponsive to activators of the NF-kB or … WebApr 3, 2014 · In practice, the term "promoter" describes the combination of the promoter (RNA polymerase binding site) and operators (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target genes. The sequence of the promoter region controls the binding of the RNA polymerase and transcription factors, … ffmc47