Fitc labeled collagen
WebMay 15, 2016 · In order to develop novel diagnosis and therapies for collagen-related diseases, it is essential to construct simple and efficient methods to detect unfolded collagen fragments. We therefore have designed a FITC-labeled collagen mimic triple helical peptide, whose adsorption on the surface of GO effectively quenches its … WebMar 1, 2024 · (a) Fluorescence microscopy analysis showing the colocalization of FITC-labeled CNA35 (green; upon incubation of tissue sections) with collagen fibers of type I, III, and IV (Col I, Col II, and Col III, respectively; all in red) on renal tissue sections from healthy mice and mice with unilateral ureteral obstruction. Nuclei are counterstained ...
Fitc labeled collagen
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WebFeb 1, 2008 · FITC labeled chitosan (FITC-Chi) and rhodamine labeled collagen (Rd-Col) were used to prepare electrospun membranes for detection of the presence of each biopolymer in the nanofiber [14]. The image of Rd-Col and FITC-Chi was obtained separately from confocal laser scanning microscopy (CLSM, Leica TCS SP2) where … WebCollagen is a major component of the extracellular matrix in vertebrates and it constitutes approximately 25% of total protein. It plays an important in cell adhesion and migration. Specific collagen receptors, fibronectin and a …
WebFITC-Labeled Type I and Type II Collagen. FITC-labeled bovine type I and type II collagen (conjugated at a 1:1 ratio) are excellent substrates for examining collagenase activity. To minimize background values in MMP … WebMar 28, 2024 · The excessive Cy5-CpG 1826 was removed by washing with PBS for 3 times before the addition of FITC labeled PDA NPs (50 μg/mL) or not. After incubation (24 h), the cells were stained with Lyso- Tracker Red (Beyotime, China) and DAPI for observation by confocal microscope (Leica SP8, Germany).
WebApr 5, 2024 · Collagen-loaded liposomes were prepared by a combination of film dispersion method and ultrasonication. 240 mg egg yolk lecithin dissolved in anhydrous ethanol was …
WebNov 18, 2024 · The next day a 50 μg/ml stock of FITC-Col was prepared by mixing 500 μL of collagen type 1-FITC (1 mg/mL Chondrex cat# 4001) with 9.5 mL media, incubating 20 …
WebCollagen type I, FITC conjugate, is supplied as a solution in 0.01 M acetic acid. The collagen concentration is 1 mg/ml. Precautions and Disclaimer The country of origin of the bovine starting material is the United States. Preparation Instructions Collagen type I, … fielding noteWebNov 15, 2024 · FITC-collagen was observed as dots in an intracellular distribution ( Fig. 1 B). To examine involvement of the autophagy–lysosome pathway in the degradation of … fielding nicholsonWebApr 5, 2024 · Fluorescein-Labeled Collagen. Fluorescein isothiocyanate (FITC) was added to 20 mL of 1 mg/mL collagen carbonate buffer (0.1 M, pH 9.0), stirred at room temperature and crosslinked at 450 rpm for 18 h in the dark. The labeled collagen solution was centrifuged at 3000 rpm for 15 min, washed five times with carbonate buffer and … fielding offers definitionWebCollagen Type I Fitc, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … fielding nurseryWebStructurally, Collagen I is a heterotrimer comprised of two alpha-1 chains and one alpha-2 chain that together forms a triple helix (1, 3, 5). Each alpha chain is primarily formed by glycine, proline, and hydroxyproline repeats with the alpha-1 chains having a theoretical molecular weight of 139 kDa and 129 kDa for the alpha-2 chain (3). grey scrabbleWebNov 18, 2024 · The next day a 50 μg/ml stock of FITC-Col was prepared by mixing 500 μL of collagen type 1-FITC (1 mg/mL Chondrex cat# 4001) with 9.5 mL media, incubating 20 min on ice, then adding 10 μL of 1 ... fielding novelistWebBoth given answers are great ideas, however I would prefer to go for FITC-labeled collagen. In my studies I used 5 wt-% (of collagen, e.g. if you have 2 g/l gels, you have a Coll-FITC conc. of 0.1 ... grey screen + citrix