WebJan 26, 2024 · After regeneration on PRMB medium containing 3 µM PABA, four-day-old transformed protoplasts were selected on BCDAT medium containing 3 µM PABA, 100 mg/l gentamicin, and 20 mg/l G418 for one week. Transformants were picked to BCDAT+PABA medium, then 10 days later a small clump of each was transferred to … WebFeb 8, 2024 · BCDAT medium was additionally supplemented with 1 mM ammonium tartrate 50. Plants were grown at 24 o C with a 16 h: 8 h, light (300mmol m -2 s -1 ): dark …
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WebAug 17, 2024 · The gametophores were then covered with solid BCDAT medium with 10 µg ml −1 PI without zeocin, and observation was resumed. Bright-field and PI fluorescence images of two or three leaves of ... WebFeb 12, 2024 · BCDAT medium : Add 10 mL of stock B, 10 mL of stock C, 10 mL of stock D, 10 mL of 500 mM ammonium tartrate, 1 mL of alternative TES, and 20 mL of 50 mM CaCl 2 to 900 mL of water, and fill to 1000 mL with water. For solid medium, add 8 g of agar per 1000 mL of medium. 3. BCDATG medium : BCDAT medium supplemented with 5 g/L … buck peeking around tree
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WebFeb 16, 2007 · For immunofluorescence, tissue grown on BCDAT or BCD medium was harvested 10 and 15 d after inoculation. Immunofluorescence Microscopy. Tissue from P. patens cultured on BCDAT or BCD medium was prepared for immunofluorescent localization of mannan as described previously (McCartney et al., 2003). Briefly, tissue … WebSep 8, 2024 · The cellophane and cultures were then transferred onto BCDAT agar medium containing various concentrations of arginine and cultured for an additional 7 days. The pH of the BCDAT medium was 6.5, but was modified to 8.5 with KOH to prepare an increased-pH control for BCDAT medium supplemented with 3.0 mM arginine. … WebMar 15, 2024 · Phenotypic analysis of WT and transgenic lines. (A) Cell lines were grown in BCDAT medium for 35 days and photographed using Miotic SMZ-171 microscope.(B) Cells grown in similar conditions as ... creed iii 2023 tickets