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Bcdat medium

WebJan 26, 2024 · After regeneration on PRMB medium containing 3 µM PABA, four-day-old transformed protoplasts were selected on BCDAT medium containing 3 µM PABA, 100 mg/l gentamicin, and 20 mg/l G418 for one week. Transformants were picked to BCDAT+PABA medium, then 10 days later a small clump of each was transferred to … WebFeb 8, 2024 · BCDAT medium was additionally supplemented with 1 mM ammonium tartrate 50. Plants were grown at 24 o C with a 16 h: 8 h, light (300mmol m -2 s -1 ): dark …

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WebAug 17, 2024 · The gametophores were then covered with solid BCDAT medium with 10 µg ml −1 PI without zeocin, and observation was resumed. Bright-field and PI fluorescence images of two or three leaves of ... WebFeb 12, 2024 · BCDAT medium : Add 10 mL of stock B, 10 mL of stock C, 10 mL of stock D, 10 mL of 500 mM ammonium tartrate, 1 mL of alternative TES, and 20 mL of 50 mM CaCl 2 to 900 mL of water, and fill to 1000 mL with water. For solid medium, add 8 g of agar per 1000 mL of medium. 3. BCDATG medium : BCDAT medium supplemented with 5 g/L … buck peeking around tree https://tycorp.net

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WebFeb 16, 2007 · For immunofluorescence, tissue grown on BCDAT or BCD medium was harvested 10 and 15 d after inoculation. Immunofluorescence Microscopy. Tissue from P. patens cultured on BCDAT or BCD medium was prepared for immunofluorescent localization of mannan as described previously (McCartney et al., 2003). Briefly, tissue … WebSep 8, 2024 · The cellophane and cultures were then transferred onto BCDAT agar medium containing various concentrations of arginine and cultured for an additional 7 days. The pH of the BCDAT medium was 6.5, but was modified to 8.5 with KOH to prepare an increased-pH control for BCDAT medium supplemented with 3.0 mM arginine. … WebMar 15, 2024 · Phenotypic analysis of WT and transgenic lines. (A) Cell lines were grown in BCDAT medium for 35 days and photographed using Miotic SMZ-171 microscope.(B) Cells grown in similar conditions as ... creed iii 2023 tickets

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Bcdat medium

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WebJul 3, 2024 · Selection plates are prepared by melting BCDAT medium, cooling to 55 °C, adding antibiotic (e.g., 15 μg/mL hygromycin), and pouring about 25 mL each into … WebJun 1, 2016 · The body plan of the early-diverged moss Physcomitrella patens (P. patens) is relatively simple. The vegetative gametophyte is composed of only a few cell types and developmental stages. Auxin was …

Bcdat medium

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WebOn minimal BCD medium, protonemal growth was strongly reduced and eventually ceased after a month (Figure 2A, C and G). At this stage, colonies comprised both chloronemal and caulonemal cells and... WebMay 25, 2024 · BCDAT medium: BCD medium supplemented with 5 mM diammonium (+)-tartrate. 3. BCDATG medium: BCDAT medium supplemented with 0.5% glucose. 2.4 Solutions and Media for Transformation 1. 8% Mannitol: Dissolve 8 g D-mannitol in 100 ml H 2 O and sterilize by autoclaving. Store at room temperature. 2.

WebFeb 6, 2024 · Protonemata were dispersed in liquid BCDAT medium containing bleomycin or MMS and treated for 1 h. After treatment and washing recovered protonemata were inoculated as eight explants per quadrant of a Petri dish with drug-free BCDAT agar without cellophane overlay and left to grow. Web, 0.17 mM KI) agar medium supplemented with 1mM CaCl 2 and 5mM ammonium tartrate (BCDAT medium), or as lawns of protonemal filaments by subculture of homoge-nized tissue on BCDAT agar medium overlaid with cellophane for the isolation of protoplasts. Bacterial strains and constructs Plasmid Tnk23 (Lucas etal., 1995) is a derivative of the …

WebDec 12, 2024 · Three-week-old protonemata of WT, ko lines and complemented lines grown on BCDAT medium were observed using a light microscope. Phenotypes of protonemal cells were observed in WT, ko lines and complemented lines. (a) ppkp4-1, ppkp4-2 and ppkp4-1/2 knockout mutants showed dead cells in protonemata. WebAfter bleomycin treatment, tissue was thoroughly rinsed in water, blotted on filter paper and either flash-frozen in liquid N 2 (repair t = 0) or left to recover on plates in liquid BCDAT medium ...

WebFeb 8, 2024 · BCDAT medium was additionally supplemented with 1 mM ammonium tartrate 50. Plants were grown at 24 o C with a 16 h: 8 h, light (300mmol m-2 s-1): dark cycle. To induce sporophytes, tissues were grown for 3-6 months on peat plugs at 16°C with an 8 h: 16 h, light (300mmol m-2 s-1): dark cycle. Sporangia were sterilized in 20% …

WebNov 18, 2024 · BCDAT medium is composed of 1 mM MgSO 4, 1.84 mM KH 2 PO 4 (pH 6.5), 10 mM KNO 3, 45 μM FeSO 4, trace element solution (0.22 μM CuSO 4, 10 μM H 3 … creed iii budgetWebDec 7, 2015 · Transformants were confirmed after three rounds of selection on BCDAT medium supplemented with G418 medium (30 μg·mL −1) alternatively for 15 days each … buck pedestal mountWebApr 8, 2024 · patens protonemal tissue was grown on BCDAT medium with and without β-estradiol for 2 days and total RNA was isolated using Trizol and a RNeasy mini kit … creed iii box office mojoWebBCDATG or BCDAT medium is used for sub-culture of P. patens protonemata. Protonemata for PEG-mediated transformation are sub-cultured on BCDATG agar … buck pearl sWebAll strains were cultured either as 'spot inocula' on BCD agar medium supplemented with 1 mM CaCl 2 and 5 mM ammonium tartrate (BCDAT medium) or as lawns of protonema … creed iii assistir onlinebuck pension log inWebJun 1, 2024 · Finally, half of the gametophores of S0, S1 and R1 were used for total RNA extraction, and half of R1 gametophores were transferred to BCDAT medium for continued growth. Arabidopsis thaliana ( A. thaliana ) seeds, ecotype Col-0 were washed with 75% Ethanol for 5 min, 5% sodium hypochlorite for 10 min, then washed with water for 10 … creed iii box office earnings